SuperHotTaq Gold DNA Polymerase for Real Time PCR. A special monoclonal antibody and aptamers suppress the reaction at room temperature until after the first
denaturation step and after the PCR reaction. This prevents primer-dimers and other nonspecific amplifications. Using the enzyme there is no need to adjust the existing standard PCR
protocol. SuperHotTaq Gold DNA Polymerase possesses a 5’ - 3’ polymerase activity and generates 3’A-overhangs.
- Hot Start PCR / qPCR (Multiplex-, Low Copy PCR; tested for PCR
with dual-labeled probes or intercalating dyes)
- Suitable for one-step RT-PCR, because of two types of inhibitors;
reversible Hot-Start features
- Suitable for test kits with pre-amplification
- enhanced PCR sensitivity up to 5 kb fragment-length
Gold DNA is a composition of a high purified and processive Taq DNA Polymerase and a mixture of monoclonal antibodies and low-molecular weight aptamers.
The Polymerase is inactive at
room temperature and for about 20-25 minutes at 55°C (e.g. for a RT-PCR). Starting from the first denaturation the enzyme is activated for PCR. The monoclonal antibody is dissolving immediately.
After PCR the polymerase restores its re-inactivation due to the aptamer.
Concentration: 5 u/µl and up to 20 u/µl on request
unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 °C.
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA; 1 mM DTT, 50 % Glycerol, 0.5 % Nonident P-40, 0.5 % Tween-20
Reaction Buffer (provided):
- Reaction buffer (10X)” incomplete” (red cap):160 mM
670mM TrisHCl pH8,8, 0,1% Tween-20
- Reaction buffer (10X) “complete” (yellow cap):160 mM (NH4)2SO4,
670mM TrisHCl pH8,8, 0,1% Tween-20, 25mM MgCl2
- separate Tube: MgCl2 (100 mM, green cap)
Transportation: Taq DNA polymerase is send on blue ice
Storage: at -20°C for 24 months or for more than 3 months at +4°C
Activity and performance test, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test, Lot-stability