Superhot Taq >GOLD< DNA Polymerase

Features:

SuperHotTaq Gold DNA Polymerase for Real Time PCR. A special monoclonal antibody and aptamers suppress the reaction at room temperature until after the first denaturation step and after the PCR reaction. This prevents primer-dimers and other nonspecific amplifications. Using the enzyme there is no need to adjust the existing standard PCR protocol. SuperHotTaq Gold DNA Polymerase possesses a 5’ - 3’ polymerase activity and generates 3’A-overhangs.

Applications: 
- Hot Start PCR / qPCR (Multiplex-, Low Copy PCR; tested for PCR
  with dual-labeled probes or intercalating dyes)
- Suitable for one-step RT-PCR, because of two types of inhibitors;
  reversible Hot-Start features
- Suitable for test kits with pre-amplification
- enhanced PCR sensitivity up to 5 kb fragment-length

Description:

SuperHotTaq Gold DNA is a composition of a high purified and processive Taq DNA Polymerase and a mixture of monoclonal antibodies and low-molecular weight aptamers.

The Polymerase is inactive at room temperature and for about 20-25 minutes at 55°C (e.g. for a RT-PCR). Starting from the first denaturation the enzyme is activated for PCR. The monoclonal antibody is dissolving immediately. After PCR the polymerase restores its re-inactivation due to the aptamer.

Concentration: 5 u/µl and up to 20 u/µl on request

Unit definition:

One unit of activity is defined as the amount of enzyme required to incorporate 10 nmoles of dNTP into acid-insoluble DNA fraction in 30 minutes at 72 °C.

Storage Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA; 1 mM DTT, 50 % Glycerol, 0.5 % Nonident P-40, 0.5 % Tween-20

Reaction Buffer (provided):
- Reaction buffer (10X)” incomplete” (red cap):160 mM (NH₄)₂SO₄,
  670mM TrisHCl pH8,8, 0,1% Tween-20
- Reaction buffer (10X) “complete” (yellow cap):160 mM (NH₄)₂SO₄,
  670mM TrisHCl pH8,8, 0,1% Tween-20, 25mM MgCl₂
- separate Tube: MgCl₂ (100 mM, green cap)

Transportation: Taq DNA polymerase is send on blue ice

Storage: at -20°C for 24 months or  for more than 3 months at +4°C

Activity and performance test, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test, Lot-stability 

Use your existing and optimized protocol for standard DNA polymerase. In contrast to chemically modified Taq DNA pol. where the first denaturation step needs up to 15 min, SuperhotTaq Gold for Real Time PCR does not need a prolonged heating or denaturation time and works excellent basis enzyme for RT-PCR

General Thermo-Cycler protocol:

Note: 
- vortex all solutions carefully before using
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .