Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Protein Electrophoresis
The Protein ladder PS10 PLUS is a mixture of 10 purified proteins which are re-dissolved 'ready-to-use' in loading buffer. The proteins resolve into 10 sharp bands in the range of 11-180 kDa when analysed by SDS-PAGE and stained with optimized dyes. The marker is ready-to-use. There is no need to boil.
• Monitoring of protein migration during SDS-polyacrylamide gel
• Monitoring of protein transfer onto membranes during Western
• Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Mini gel application: 10 μl/well; 5 µl per well blots
Standard gel application: 20 μl/well; 10 µl per well for blots
Number of bands: 10 11, 17, 25, 35, 48, 63, 75, 100,135, 180 kDa
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
- Thaw marker at room temperature or heat at 37 – 40 °C for a few minutes. Do not boil!
- Vortex gently to ensure the solution is homogeneous and load the ladder on SDS-polyacrylamide gel
- 10 μl per well for mini-gels, 5 μl per well for blots
- 20 μl per well for large gels, 10 μl per well for blots
1 ml Protein ladder is sufficient for 100 mini gels or 50 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.
Protein ladder PS10 PLUS is optimized for runs on 12 % SDS polyacrylamide gels. 8 to 10 % gels may cause proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage: at -20°C
Shipment: on blue ice
Protein Molecular Weight Marker PS10 (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting.
* availibility of sample size may be limited
Final price excl. shipping costs3
Dear customer, GeneON likes to send free samples to convince the valued customer about the quality. Please understand that the shipping costs may be very high to some destinations. That is the reason why we cannot assure to fulfil all sample requests. Sorry for that, please understand. You may also set an inquiry/order directly by e-mail .