Features:
- The Master mix contains dTTP
- The Mix contains High-ROX as passive
   Reference dye (it provides a baseline in multiplex reactions)
- It contains EvaGreen as fluorescent dye
- The qPCR / RTD-PCR Master mix E3 is ready-to-use and is optimized
  for high specificity and sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix

Compatibility:
ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus

Applications:

- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Description:
The PCR-Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The PCR-mix for qPCR offers dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions. 

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase (anti-body blocked) for qPCR, dATP, dCTP, dGTP, dTTP, EvaGreen, High-ROX, optimized reaction buffer with KCl and MgCl₂, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

Manufactured and quality-controlled in accordance with ISO 9001:2000

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
  necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol for qPCR / RTD-PCR Master mix:

Note: working with EvaGreen just select the optical setting for FAM or SYBR Green at the cycler

Note: 
an individual optimization of annealing temperature may be necessary for new combinations of primers and Template DNA