Real-time-PCR Master Mix E3

The PCR Master mix for qPCR contains: EvaGreen, dUTP and high ROX (500 nM)

Features:
- The Master mix contains dUTP instead of dTTP
- The Mix contains ROX (500nM; LOW-ROX-version 100mM) as passive
Reference dye (it provides a baseline in multiplex reactions)
- It contains EvaGreen as fluorescent dye
- The qPCR / RTD-PCR Master mix E3 is ready-to-use and is optimized
for high specificity and sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix

Compatibility:
500 nM Version: ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus
100 nM Version: 7500 System, Stratagene Mx35005P, Mx4000 and Mx3000P, ViiA 7, QuantStudio

Applications:

- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, EvaGreen, ROX, optimized reaction buffer with KCl and MgCl₂, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

Note: protect from Light

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001:2000

PCR master mix for Realtime PCR with EvaGren and ROX content. How to use.

Components	Volume per reaction	final conc.
2X qPCR / RTD- PCR Master mix E3	25 µl	1x
Up-stream primer (10 µM stock)	1,5 µl (range: 0,5-2.5 µl)	300 nM
Down-stream primer (10µM stock)	1,5 µl (range: 0.5-2,5 µl	300 nM
Template DNA	5 µl (0.1-15 ng/ml plasmid DNA) (1-10 µg/ml genomic DNA)	< 500ng DNA
Sterile dest. Water (included)	up to 50 µl total reaction volume

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol for qPCR / RTD-PCR Master mix:

Note: working with EvaGreen just select the optical setting for FAM or SYBR Green at the cycler

Step	Time	Temperature
Initial denaturation	1-3 min	95°C
30-40 Cycles: Denaturation Annealing Extension	15-30 sec 30-65 sec 30 sec (per 500bp)	95°C 55-65°C 72-75°C

Note:
an individual optimization of annealing temperature may be necessary for new combinations of primers and Template DNA

Real-time-PCR Master Mix E3

The Master mix for qPCR contains: EvaGreen, dUTP and ROX (500 nM)

* availibility of sample size may be limited

129,00 € ²

115,00 €

order / kaufen

Final price excl. shipping costs³

verfügbar / avaílable
1 - 3 Tage Lieferzeit / 1-3 days delivery¹

Related products

Ultra pure Nucleotides

PCR MASTERMIX SELECTION TABLE

PCR Mastermixes with dTTP and SybrGreen

BioStar 2 PCR mastermix with SYBRGreen, blue dye, no rox

BioStar 3 PCR mastermix with SYBRGreen, 60 nm LOW-ROX

BioStar 4 PCR mastermix with SYBRGreen, 900 nm HIGH-ROX

PCR Mastermixes with dTTP for probes

BioStar 5 PCR mastermix for probes

BioStar 6 PCR mastermix for probes 60 mM LOW-ROX

BioStar 7 PCR mastermix for probes 900 mM HIGH-ROX

PCR Master Mixes with Evagreen and dUTP

PCR Master Mix for qPCR EVA 1 dUTP+ EvaGreen

PCR Master Mix for qPCR EVA 2 +UNG

PCR Master Mix for qPCR EVA3 +ROX

PCR Master Mix for qPCR EVA4 +ROX+UNG

PCR Master Mix for qPCR EVA5 +low ROX

PCR Master Mix for qPCR E6 +low ROX+UNG

PCR Master Mixes for probes and dUTP

qPCR Master Mix DLP1 Exclusiv +dUTP

qPCR Master Mix DLP2 Exclusiv +dUTP+UDG

qPCR Master Mix DLP3/DLP5 Exclusiv ROX (500 nM, 100nM)

qPCR Master Mix DLP4/DLP6 Exclusiv +ROX 500nM or 100 nM+UDG

Deutsche Beschreibung

qPCR Mastermix E3

Datasheet qPCR-Mastermix E3

Material Safety Datasheet

References / Protocols / Notes / Recomendations / Tests

¹ Applies to shipping within Germany. Information about shipping policies for other countries can be found here: Payment and Delivery Information
² limitierter Sonderpreis / Special Price limited
³ Nettopreise ohne Umsatzsteuer zzgl. Frachtkosten / All prices without VAT excl. freight costs. :: MUSTER FÜR DEUTSCHLAND WERDEN KOSTENFREI GELIEFERT - AUCH WENN IM SHOP EIN PREIS AUSGEWIESEN WIRD :::

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Real-time-PCR Master Mix E3

The PCR Master mix for qPCR contains: EvaGreen, dUTP and high ROX (500 nM)

Stability tests / Quality control / Comparison

PCR master mix for Realtime PCR with EvaGren and ROX content. How to use.

The Master mix for qPCR contains: EvaGreen, dUTP and ROX (500 nM)