Real-time-PCR Master Mix E4

The PCR-Master mix for qPCR contains: EvaGreen, dUTP. UNG and high ROX (500 nM)

Features:
- The PCR-Master mix contains dUTP instead of dTTP
- The MasterMix for PCR contains UNG (Uracil-N-Glycosylase)
- The PCR-Mix contains ROX (500nM or LOW-ROX-version 100nM) as
  passive Reference dye (it provides a baseline in multiplex reactions)
- It contains EvaGreen as fluorescent dye
- The qPCR / RTD-PCR Master mix E4 is ready-to-use and is optimized
  for high specificity and sensitivity because of optimized reaction buffer
- easy to use because ready-to-use Master Mix

Applications:
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Compatibility:
500 nM Version: ABI: 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast, StepOne, StepOne plus
100 nM Version: 7500 System, Stratagene Mx35005P, Mx4000 and Mx3000P, ViiA 7, QuantStudio

Description:
The PCR-Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The PCR-mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions. 

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix:
Hot-Start Polymerase for qPCR, dATP, dCTP, dGTP, dUTP, EvaGreen, ROX, UNG, optimized reaction buffer with KCl and MgCl2, stabilizers and enhancers, PCR-grade water

Transportation: with blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months,

 

Note: protect from Light

 

Stability tests / Quality control / Comparison

Manufactured and quality-controlled in accordance with ISO 9001 and 14001

PCR Mix: How to use.

 

 Components  Volume per reaction  final conc.
 2X qPCR / RTD-
 PCR Master mix E4
 25 µl  1x
 Up-stream primer (10
 µM stock)
 1,5 µl (range: 0,5-2.5 µl)  300 nM
 Down-stream primer
 (10µM stock) 
 1,5 µl (range: 0.5-2,5 µl  300 nM
 Template DNA  5 µl
 (0.1-15 ng/ml plasmid DNA)
 (1-10 µg/ml genomic DNA)
 < 500ng
 DNA

 Sterile dest. Water (included)

 up to 50 µl total reaction volume  


- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be
  necessary for new combinations of primers and Template DNA

General Thermo-Cycler protocol for qPCR / RTD-PCR Master mix:

Note: working with EvaGreen just select the optical setting for FAM or SYBR Green at the cycler

 

 Step  Time  Temperature
 Initial denaturation  1-3 min  95°C
 
30-40 Cycles:
 Denaturation
 Annealing
 Extension
 
 

 15-30 sec
 30-65 sec
 30 sec 
 (per 500bp)

 
 95°C
 55-65°C
 72-75°C 
 


Note: 
an individual optimization of annealing temperature may be necessary for new combinations of primers and Template DNA

 

 

Real-time-PCR Master Mix E4 (2x); qPCR Mastermix

The Master mix for qPCR contains: EvaGreen, dUTP. UNG and ROX (500 nM).

The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.
The mix offer dUTP instead of dTTP to prevent carry-over contaminations of DNA from previous PCR reactions. 

* availibility of sample size may be limited

169,00 € 2
140,00 €
order / kaufen
  • verfügbar / avaílable
  • 1 - 3 Tage Lieferzeit / 1-3 days delivery1

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Ultra pure Nucleotides

PCR MASTERMIX SELECTION TABLE

 

PCR Mastermixes with dTTP and SybrGreen

BioStar 2 PCR mastermix with SYBRGreen, blue dye, no rox

BioStar 3 PCR mastermix with SYBRGreen, 60 nm LOW-ROX

BioStar 4 PCR mastermix with SYBRGreen, 900 nm HIGH-ROX

 

PCR Mastermixes with dTTP for probes

BioStar 5 PCR mastermix for probes

BioStar 6 PCR mastermix for probes 60 mM LOW-ROX

BioStar 7 PCR mastermix for probes 900 mM HIGH-ROX

 

PCR Master Mixes with Evagreen and dUTP

PCR Master Mix for qPCR EVA 1 dUTP+ EvaGreen

PCR Master Mix for qPCR EVA 2 +UNG

PCR Master Mix for qPCR EVA3 +ROX

PCR Master Mix for qPCR EVA4 +ROX+UNG

PCR Master Mix for qPCR EVA5 +low ROX

PCR Master Mix for qPCR E6 +low ROX+UNG

 

PCR Master Mixes for probes and dUTP

qPCR Master Mix DLP1 Exclusiv +dUTP

qPCR Master Mix DLP2 Exclusiv +dUTP+UDG

qPCR Master Mix DLP3/DLP5 Exclusiv ROX (500 nM, 100nM)

qPCR Master Mix DLP4/DLP6 Exclusiv +ROX 500nM or 100 nM+UDG

 


Deutsche Beschreibung

 

qPCR Mastermix E4

Datasheet qPCR-Mastermix E4

Material Safety Datasheet


References / Protocols / Notes / Recomendations / Tests

 

 

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