AspA2 I - C↑CTAGG - GGATC↓C

Source: Arthrobacter species A2

 

 

 

 

 

 

  

Recognition site:

C↑CTAGG

GGATC↓C

 

Source: Arthrobacter species A2

 

Assayed on : Lambda DNA (HindIII-digest)

 

Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Optimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA

                     

Activity in SEBuffers:

SEBuffer B       10 - 25%

SEBuffer G       50 - 75%

SEBuffer O       25 - 50%

SEBuffer W            100%

SEBuffer Y      75 - 100%

SEBuffer ROSE     100%

 

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

 

Optimal temperature: 37°C

 

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.

 

Store at -20°C.

 

Ligation: After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

 

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.

 

Reagents Supplied with Enzyme: 10 X SE-buffer W, BSA

 

Methylation sensitivity: not tested

 

Inactivation: 20 minutes under 80°C

 

Notes: To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.

 

E246 - AspA2 I - 2500 units

AspA2 I - C↑CTAGG - GGATC↓C -  Restriction Endnuclease from Sibenzyme

 

* availibility of sample size may be limited

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References / Protocols / Notes / Recomendations / Tests

 

 

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