BseP I - G↑CGCGC - CGCGC↓G

Source: Bacillus stearothermophilus P

 

 

 

 

 

 

 

Recognition site:

G↑CGCGC

CGCGC↓G

 

Source: Bacillus stearothermophilus P

 

Assayed on: Lambda DNA

 

Unit definition: One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.

 

Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

 

Activity in SEBuffers:

SEBuffer B         50 - 75%

SEBuffer G               100%

SEBuffer O       75 - 100%

SEBuffer W       50 -  75%

SEBuffer Y        50 -  75%

SEBuffer ROSE       100%

 

When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion. 

 

Optimal temperature: 50°C

 

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol;

 

Store: at -20°C.

 

Ligation: After 5-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.

 

Non-specific hydrolisis: No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.

 

Reagents Supplied with Enzyme: 10 X SE-buffer G

 

Methylation sensitivity: Blocked by CG methylation.

 

Inactivation: 20 minutes under 65°C

 

E182 - BseP I - 1000 units

BseP I - G↑CGCGC - CGCGC↓G - Restriction Endnuclease from Sibenzyme

 

* availibility of sample size may be limited

168,00 €
order / kaufen
  • 5 - 8 Tage Lieferzeit1

Related products

 

 

 

 

 

Ultra pure Nucleotides


Datasheet

Material Safety Datasheet


References / Protocols / Notes / Recomendations / Tests

 

 

Matrix themes

Close