The one-step-kit contains all reagents required for RT-qPCR in a bundle to ensure fast and easy preparation with a minimum of pipetting steps. Just add template, primers. That's all.
The mix is coloured for easy pipetting
High sensitivity (1pg – 1 µg of RNA)
Contains an inhibitor of RNases
error-correcting activity because of Pfu DNA Polymerase
recommended for GC-rich templates
templates up to 5 kb
Bio-Star Master-mix contains the optimal ratio of M-MuLV–RH to HS-Taq DNA polymerase for both reactions.
M-MuLV–RH is a genetically modified Moloney Murine Leukemia Virus reverse transcriptase (M-MuLV). The enzyme has RNA- and DNA-dependent polymerase activity but lacks RNase H activity.
M-MuLV–RH reverse transcriptase exhibits improved thermal stability and is active at high temperatures (up to 50°С).
HS-Taq DNA polymerase is a recombinant Taq DNA polymerase inactivated by specific monoclonal antibodies.
The enzyme is inactive at temperatures up to 70 °C, it is activated at the first PCR cycle during a short 5-min incubation at 95 °C. Recombinant HS-Taq DNA polymerase catalyses 5’→3’ synthesis of
DNA and possesses 5’→3’ exonuclease activity of the native Taq DNA polymerase from Thermus aquaticus.
Recombinant HS-Taq DNA polymerase is ideal for conventional PCR of the templates of up to 5 kbp.
- Gene expression analysis;
- One-step real-time RT-qPCR.
1. BioStar Enzyme-mix:
M-MuLV–RH reverse transcriptase, mixture of HS-Taq DNA Polymerase (inhibited with mAB), Pfu DNA Polymerase and inhibitor of RNases, stabilizer and enhancer
2.) Re-Action buffer (2X):
100 mM Tris-НCl (рН 8.3 at 25 °С), 150 mM KCl, 0.6 mM each deoxynucleoside triphosphate, 6 mM MgCl2, 8 mM DTT, fluorescent dye SYBR Green I and inert dye, stabilizer
The absorption maximum of the inert dye is 615 nm.
@ -20°C, short term at room temperature up to 2-3 days.
@ +4°C for about one week.
About 30 times Multiple thawing/freezing without any loss of activity.
the product will be shipped with "blue ice"; up to 2-3 days @ room temperature.