The kit contains all reagents required for RT-qPCR in a set to ensure fast and easy preparation with a minimum of pipetting steps. Just
add template, primers. That's all.
The One.Step RT-PCR with UNG Kit is highly sensitive: less than 10 pg to 1 µg total RNA or < 1 pg to 20 ng poly(A) RNA (mRNA) can be detected when using
highly expressed transcripts
The enzyme mix is based on a new formulated reverse transcriptase, a Hot-Start Polymerase (mAB), UNG and RNase Inhibitor. The set is providing
increased specificity, high cDNA yield and improved efficiency for highly structured and long cDNA fragments. The reaction buffer includes extrapure dNTPs (dUTP), and reaction
In the RT-step the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA) complementary to the RNA template. The
hot-start polymerase activity is blocked.
In the first cycle of the PCR step the Hot-Start DNA polymerase activity is switched on and
it synthesizes DNA molecules complementary to the cDNA, thus generating a double-stranded DNA template.
The One.step Realtime RT-PCR Master Mix from GeneON offers a tremendous convenience
when applied to analysis of targets from multiple samples of RNA and minimizes the risk of contaminations.
That Mastermix is validated for the diagnosis of SARS-CoV-2 causing of the new Corona
Platforms: The Kit is suitable for all block-based Thermocycler. Stringent Quality Tests on ABI StepOne plus PCR Cycler
Components of Maximo.OneStep RT-qPCR:
Enzyme-mix: HotStart Taq Polymerase with mAB, Reverse Transcriptase, RNase Inhibitor and
enhancers, Reaction buffer, UNG, dNTPs incl. dUTP, and stabilizers
@ -20°C, avoid frequent thawing and freezing,
the product will be shipped with "blue ice"
Manufactured and quality-controlled in accordance with ISO