The microTotal RNA Extraction Kit is designed for rapid and efficient purification of RNA from various types of samples including animal tissues, cultured cells (monolayer cells, suspension
cells), blood, plasma, serum, buffy coat, biological fluids like saliva and semen. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins
and subsequently release of RNA. Special buffers provided in the kit are optimized to enhance binding of RNA onto a specially-treated glass filter membrane for efficient recovery of highly total
RNA. Simple centrifugation steps allow multiples sample processing and remove contaminants to yield highly pure total RNA which is suitable to be used in various downstream applications.
- Purify large RNA, siRNA and microRNA
- Yields up to 3µg of total RNA
- No organic-based extraction required
- Highly pure RNA ready to use for RT-PCR, Northern Blotting, polyA RNA
(mRNA) purification, transcription profiling, nuclease protection and in
Application and components
For purification of total RNA .
- Buffer TS
- Wash Buffer (concentrate)
- RNase-free Water