The GF-1 Tissue DNA Extraction Kit is designed for rapid and efficient purification of genomic DNA from various tissue samples
such as kidney, heart, lungs, brain, muscles, liver, spleen, etc. The purification is based on the usage of denaturing agents to provide lysis of tissue cells, denaturation of proteins and
subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of
highly pure genomic DNA.
Features Tissue DNA Isolation kit:
- Yields up to 20µg of DNA
- Mini-column spin technology
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, -
Southern blotting, DNA fingerprinting, etc.
For high quality tissue genomic DNA isolation.
The GF-1 Tissue DNA Extraction Kit is optimized to isolate up to 20μg of DNA from up to 5 x 106 cultured animal cells or
10 - 20mg of tissue samples. Tissue samples vary in the number of cells depending on age, type of tissue and origin. When processing samples, do not use more than the
recommended starting material as excessive number of cells will overload the column. This would
result in reduced yield and purity. We recommend weighing the tissue samples before starting to ensure optimum yield and purity is obtained. Liver and spleen are very high in protein and
content. Thus, when isolating genomic DNA from these sources, use only up to 15mg of the sample.