10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA;
200 µg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.
1X SEBuffer Y+BSA
Incubate at 37°C.
Warranty period for the enzyme storage at-20˚C is two years from the date of the last assay
indicated on the enzyme vial.
1X SEBuffer Y (pH 7.9 @ 25ºC)
33 mM Tris-Ac, 66 mM KAc, 10 mM MgAc, 1 mM DTT
One unit is defined as the amount of enzyme required to digest 1 µg of l DNA in 1
hour at 37°C in a total reaction volume of 50 µl.
Concentrated enzymes are diluted to approximately
1000 units/ml with the buffer A [10 mM Tris-HCl (pH 7.6); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT;
200 µg/ml BSA; 50% glycerol] before determining their activity.
To obtain 100% activity, BSA should be added to the 1X reaction mix to a final concentration
of 100 µg/ml.