Quality Control Assays
10 mM Tris-HCl (pH 7.5); 100 mM NaCl;
0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
1X SEBuffer Bis I
Incubate at 37°C.
Warranty period for the enzyme storage at -20˚C is two
from the date of the last assay indicated on the enzyme
BisI (pH 9.0 @ 25ºC)
10 mM Tris-HCl 150 mM KCl
10 mM MgCl2 1 mM DTT
One unit is defined as the amount of enzyme required to cleave
1 pmol of the double-stranded oligonucleotide with the following structure
5' GCTTGTACTTTAG(5mC)GGCATTGATTCTCACCACG 3'
3' CGAACATGAAATCGC(5mC)GTAACTAAGAGTGGTGC 5'
in 1 hour at 37°C in a total reaction volume of 20 μl.
Reagents Supplied with Enzyme:
10X SEBuffer Bis I
Heat Inactivation: Yes (65C for 20 minutes)