1000 - 2000 u/ml Store at -20°C
Source: Flavobacterium aquatile B15
Fai I cleaves 4 expected recognition sites as well as several other sites with a weaker activity.
In the case of long incubation with Fai I DNA can be digested to small oligos.
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA; 200 µg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.
1X SEBuffer B.
Incubate at 50°C.
Warranty period for the enzyme storage at -20˚C is two years from the date of the last assay indicated on the enzyme vial.
1X SEBuffer B (pH 7.6 @ 25ºC)
10 mM Tris-HCl, 10 mM MgCl2,1 mM DTT
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double stranded oligonucleotide with the following structure
in 1 hour at 50°C in a total reaction volume of 20 μl.
Quality Control Assays
Ligation: After 3-fold overdigestion with Fai I, ~50% of pUC19 DNA fragments can be ligated with T4 DNA Ligase and recut.
No detectable degradation of a single- and double-stranded oligonucleotide was observed after incubation with 1 unit of enzyme for 3 hours.
Activity in SEBuffers:
SEBuffer B 100%
SEBuffer G 50-75%
SEBuffer O 10-25%
SEBuffer W 25-50%
SEBuffer Y 25-50%
SEBuffer ROSE 100%
Yes (80°C for 20 minutes)
Reagents supplied with enzyme:
10X SEBuffer B
Final price excl. shipping costs3