10 mM Tris-HCl (pH 7.5); 100 mM KCl;
0,1 mM EDTA;
200 µg/ml BSA;
2-mercaptoethanol; 50% glycerol.
1X SEBuffer B.
Incubate at 50°C.
Warranty period for the enzyme storage at -20˚C is two
from the date of the last assay indicated on the enzyme
1X SEBuffer B (pH 7.6 @ 25ºC)
10 mM Tris-HCl 10 mM MgCl2 1 mM DTT
One unit is defined as the amount of enzyme required
to cleave 1 pmol of the double-stranded oligonucleotide
with the following structure
in 1 hour at 50°C in a total reaction volume of 20 μl.
Ligation: After 3-fold overdigestion with
Fai I, ~50% of pUC19 DNA fragments can be
ligated with T4 DNA Ligase and recut.
No detectable degradation of a single-
and double-stranded oligonucleotide was
observed after incubation with 1 unit of
enzyme for 3 hours.