5 000 u/ml
5’… ASST* …3’
Source: An E.coli strain that carries the cloned Set I gene from Streptomyces werraensis 37
10 mM Tris-HCl (pH 7.6); 100 mM NaCl;
0,1 mM EDTA; 50% glycerol.
Incubate at 55 °C.
Warranty period for the enzyme storage at -20˚C is one year
from the date of the last assay indicated on the enzyme vial.
33 mM Tris-Ac (pH 7.9 @ 25ºC) 66 mM KAc
10 mM MgAc 1 mM DTT
One unit is defined as the amount of enzyme required to cleave 1 pmol of the double-stranded oligonucleotide
with the following structure
in 1 hour at 55 ºC in a total reaction volume or 20 µl
Quality Control Assays
Ligation: After 5-fold overdigestion with
Set I, approximately 50% of DNA fragments can be
ligated with T4 DNA Ligase and recut.
*SetI cleaves a canonical site and several other sites with a weaker
In the case of long incubation with SetI DNA can be digested to small oligos.
No detectable degradation of a single- and double-stranded oligonucleotide was observed after
incubation with 5 units of enzyme for 3 hours.
SEBuffer B 25-50%
SEBuffer G 25-50%
SEBuffer O 75-100%
SEBuffer W 75-100%
SEBuffer Y 100%
SEBuffer ROSE 100%
When using a buffer other than the
optimal (suppied) SEBuffer, it may be
necessary to add more enzyme to achieve
Yes (80°C for 20
Reagents Supplied with
10X SEBuffer Y
Ultra pure Nucleotides
Agarose for perfect results
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