5000 u/ml Store at -20°C
Source: Agrococcus species 25
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0,1 mM EDTA;
200 µg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol.
1X SEBuffer Y+BSA
Incubate at 37°C.
Warranty period for the enzyme storage at-20˚C is two years from the date of the last assay indicated on the enzyme vial.
1X SEBuffer Y (pH 7.9 @ 25ºC)
33 mM Tris-Ac, 66 mM KAc, 10 mM MgAc, 1 mM DTT
One unit is defined as the amount of enzyme required to digest 1 µg of l DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Concentrated enzymes are diluted to approximately
1000 units/ml with the buffer A [10 mM Tris-HCl (pH 7.6); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol] before determining their activity.
To obtain 100% activity, BSA should be added to the 1X reaction mix to a final concentration of 100 µg/ml.
Quality Control Assays
Activity in SEBuffers:
SEBuffer B 75-100%
SEBuffer G 50- 75%
SEBuffer O 10- 25%
SEBuffer W 10- 25%
SEBuffer Y 100%
SEBuffer ROSE 50%
When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Yes (65°C for 20 minutes)
Reagents supplied with enzyme:
10X SEBuffer Y, BSA (10 mg/ml)
Restriction Endnuclease from Sibenzyme
* availibility of sample size may be limited
Final price excl. shipping costs3