Lmn I
Source: Lysinibacillus manganicus An22
500 - 1000 u/ml store at -20°C
Recognition Sequence:
GCTCCN↑
CGAG↓GN
Sourse: Lysinibacillus manganicus An22
Supplied in:
10 mM Tris-HCl (pH 7.4); 50 mM NaCl; 0,1 mM EDTA; 200 µg/ml BSA; 1 mM DTT; 50% glycerol.
Reaction Conditions:
1XSEBuffer B.
Incubate at 37°C.
1XSEBuffer B:
10 mM Tris-HCl (pH 7.6 @ 25ºC), 10 mM MgCl2, 1 mM DTT
Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Quality Control Assays
Ligation:
After 10-fold overdigestion with ZraI, approximately 90% of λ DNA fragments can be ligated with T4 DNA Ligase and recut. In the presence of 10% PEG ligation is better.
16-Hour Incubation:
A 50 µl reaction containing 1 µg of λ DNA and 10 units of enzyme incubated for 16 hours resulted in the same pattern of DNA bands
as a reaction incubated for 1 hour.
Oligonucleotide Assay:
No detectable degradation of a single- and double-stranded oligonucleotide was observed after incubation with 10 units of enzyme for 3 hours.
Enzyme properties
Activity in SEBuffers:
SEBuffer B 100%
SEBuffer G 50- 75%
SEBuffer O 25- 50%
SEBuffer W 25- 50%
SEBuffer Y 75-100%
SEBuffer ROSE 100%
When using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Heat Inactivation:
Yes (80°C for 20 minutes)
Reagents supplied with enzyme:
10XSEBuffer B
Notes:
High enzyme concentration may result in star activity. The minimum number of units that resulted in complete digestion of 1 µg of substrate DNA in 16 hours is 0,5. Zra I cleaves linear plasmid DNA at a rate 1,5-2 times higher than supercoiled plasmid DNA.
Restriction Endnuclease from Sibenzyme
* availibility of sample size may be limited
Final price excl. shipping costs3
- verfügbar / avaílable
- 1 - 3 Tage Lieferzeit / 1-3 days delivery1
Datasheet
Material Safety Datasheet
References / Protocols / Notes / Recomendations / Tests
Contact
GeneOn GmbH
Paracelsusstrasse 10
67071 Ludwigshafen am Rhein
Deutschland / Germany
Phone: +49-621-5720 864
Fax: +49-621-5724 462
Shop-Terms
AGB / Businesss Condtitions / Freight Costs / Frachtkosten für Deutschland
See Categories
- DNA/RNA Extraction
- Real-time PCR
- RT-qPCR - Master Mixes
- PCR / DNA Amplification
- RT-PCR - Reverse Transcription
- Nucleotides, dNTPs, Primers
- Marker / Gel Staining.
- Enzymes and Finechemicals
- Fluorescent Dyes / supplements
- DNA/Ladders/Standards
- PCR-96 plates and tools, Tips, Tubes, Filtration
- Restriction Endonucleases
- Devices and Tools
News & Informations
We accept payment through
New Website for our customers!
GENEON GmbH - Germany - Paracelsusstrasse 10 - 67071 Ludwigshafen - www.geneon.net - info(at)geneon.net - Tel: 0621-5720864