1000 u/ml store at -20°C
Source: Planomicrobium koreense 78k
The enzyme cleaves C5-methylated DNA and doesn't cut unmodified DNA.
PkrI cleaves DNA sequence 5`- GCNGC-3`/3`-CGNCG-5', if at least three 5-methylcytosines are present in the recognition site (N isn`t considering) .
Optimal recognition sites (100% activity):