60 mM Tris HCl (pH 7,5), 60 mM EDTA, 50 % Glycerol, Xylene Cyanole FF; Tartrazine
Room temperature, +4°C or -20°C for long term storage
Information about DNA loading Dye:
The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward
the anode at predictable rates.
The gel - loading buffer contains only one low concentration dye (bromophenol blue and xylene cyanol FF) to avoid masking the DNA Ladder fragments. But if the added dye is masking your signal
because it is running on the same high in your gel, just dilute it more.
Loading dye 306-210 suits well for the DNA samples dissolved either in water or in EDTA-containing buffer (as TE buffer).
How to predilute a DNA ladder with the loading dye?
For DNA markers, apply 0.1 µg per 1 mm of agarose gel lane width. Often 1µg of marker is used in one electrophoresis run but it depends on the size of your gel and the comb.
If DNA markers are not prediluted with the Loading dye solution, then mix: The loading buffer is 6x concentrated, that means you have to use 5 parts DNA-Loading Dye and one part DNA.
Storage and transport: @ RT or -20 °C