The loading dye increases the density of the sample and they add colour to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward
the anode at predictable rates.
In 1% agarose gels, bromophenol blue migrates with 300 bp linear double-stranded DNA fragment, whereas xylene cyanol FF migrates at approximately the same rate as linear
double-stranded DNA 4 kb length. These relationships are not significantly affected by the concentration (0.5 to 1.4%) of agarose in the gel.
Loading: The loading dye suits well for the DNA samples dissolved either in water or in EDTA-containing buffer (as TE buffer).
If DNA markers are not prediluted with the Loading dye solution, then mix: The loading buffer is 6x concentrated, that means you have to use it 5:1.
For DNA markers, apply 0.1 µg per 1 mm of agarose gel lane width. Often 1µg of marker is used in one electrophoresis run but it depends on the size of your gel and the
Preparation: 6x Loading Dye Solution deionised water at a ratio 1:1:4 for example, 5 µl 1 kb ladder : 5 µl 6x loading dye : 20 µl water. By applying
30.0 µl of this mixture, you’ll have 1.0 µg of total DNA per lane.
Storage and transport: @ RT or -20 °C